Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] (NB120-6521) (2024)

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Technical Data Protocols Calculators & Tools Key Product Details Product Summary for Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] Scientific Data Images for Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] Applications for Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] Formulation, Preparation, and Storage Background: IgG (H+L) Product Documents for Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] Product Specific Notices for Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] Protocols Spectra Viewer

Catalog # NB120-6521 | Novus Biologicals a Bio-Techne Brand
Product Datasheet / COA / SDS

Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] (NB120-6521) (1)

View Available Conjugates

Conjugate

Catalog #

Unconjugated

NB120-6520

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Biotin

NB120-6578

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Rhodamine

NB120-6522

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Texas Red

NB120-6523

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  • Product Summary
  • Scientific Data
  • Applications
  • Background
  • Product Documents
  • Specific Notices

Key Product Details

Species Reactivity

Goat

Applications

Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Donkey IgG

Concentration

LYOPH mg/ml

Product Summary for Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC]

Immunogen

Goat IgG whole molecule

See Also
Changes in maternal body composition and metabolism of dairy goats during pregnancy

Specificity

No reaction was observed against anti-papain or anti-Donkey IgG F(c).

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Description

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein and anti-Donkey Serum

Scientific Data Images for Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC]

Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] (NB120-6521) (2)

Western Blot: Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] [NB120-6521] - Western blot of Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC].Lane 1: Goat IgG.Lane 2: None.Load: 50 ng per lane.Primary antibody: None.Secondary antibody: Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] at 1:1000 for 60 min at RT.Blocking: Fluorescent blocking buffer for 30 min at RT.Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Goat IgG.Other band(s): None.

Applications for Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC]

Application

Recommended Usage

Flow Cytometry

1:500 - 1:2500

Fluorophore-linked immunosorbent assay

1:10000 - 1:50000

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Western Blot

1:1000 - 1:10000

Application Notes

This product has been tested by western blot and is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 1.0 ml deionized water (or equivalent).

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: IgG (H+L)

Antibodies, also known as immunoglobulins (Igs) are critical for immunity and are grouped into five primary classes: IgG, IgM, IgA, IgD, and IgE. The most abundant antibody isotype is immunoglobulin G (IgG) with concentrations ranging from 7.5-22 mg/ml in human serum and has a molecular weight of 150 kDa. The major effector functions of IgG include neutralization, opsonization, complement fixation and antibody dependent cell-mediated cytotoxicity (ADCC). This monomeric immunoglobulin, expressed on the surface of mature B cells, is often depicted as a Y-shape and comprised of 2 heavy chains and 2 light chains linked by disulfide bonds. The heavy chain is type gamma including subtypes gamma 1, gamma 2, gamma 3, and gamma 4 while the light chain is either a kappa or lambda chain. An IgG molecule has two antigen binding sites, each consisting of a heavy and light chain N-terminal variable domain. When combined with the constant heavy chain 1 (Ch1) and the constant light chain domains, it forms the fragment antigen-binding (Fab) region (2 per antibody). The remaining domains (Ch2-Ch4) of both heavy chains make up the Fc region and contain a site for covalently linking an enzymatic or fluorochrome probe, such as HRP or Janelia Fluor 549, for target detection and visualization (1,2,3).

The 4 IgG subclasses, sharing 95% amino acid identity, include IgG1, IgG2, IgG3, and IgG4 for humans and IgG1, IgG2a, IgG2b, and IgG3 for mice. The relative abundance of each human subclass is 60% for IgG1, 32% for IgG2, 4% for IgG3, and 4% for IgG4. In an IgG deficiency, there may be a shortage of one or more subclasses (4).

References

1. Painter RH. (1998) Encyclopedia of Immunology (Second Edition). Elsevier. 1208-1211

2. Chapter 9 - Antibodies. (2012) Immunology for Pharmacy. Mosby 70-78

3. Schroeder H, Cavacini, L. (2010) Structure and Function of Immunoglobulins. J Allergy Clin Immunol. 125(2 0 2): S41-S52. PMID: 20176268

4. Vidarsson G, Dekkers G, Rispens T. (2014) IgG subclasses and allotypes: from structure to effector functions. Front Immunol. 5:520. PMID: 25368619

Additional IgG (H+L) Products

  • All Products for IgG (H+L)
  • IgG (H+L) Secondary Antibodies

Product Documents for Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC]

Download Product Datasheets

Product Specific Notices for Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

  • Antigen Retrieval Protocol (PIER)
  • Antigen Retrieval for Frozen Sections Protocol
  • Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
  • Extracellular Membrane Flow Cytometry Protocol
  • ICC Cell Smear Protocol for Suspension Cells
  • ICC for Adherent Cells
  • Immunocytochemistry (ICC) Protocol
  • Immunocytochemistry Video Protocol
  • Intracellular Flow Cytometry Protocol Using Alcohol
  • Intracellular Flow Cytometry Protocol Using Detergents
  • Intracellular Nuclear Target Flow Cytometry Protocol Using Detergents
  • Protocol: Annexin V and PI Staining by Flow Cytometry
  • Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
  • Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
  • Sandwich ELISA (Colorimetric) – Direct Detection Protocol
  • Western Blot Protocol
  • Western Blot Troubleshooting
  • View all Protocols, Troubleshooting, Illustrated assays and Webinars.
  • Calculators
  • Tools

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

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Donkey F(ab) anti-Goat IgG (H+L) Secondary Antibody [FITC] (NB120-6521) (2024)
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